|
KMID : 0603820110170030177
|
|
Journal of Experimental & Biomedical Science
2011 Volume.17 No. 3 p.177 ~ p.184
|
|
|
Potassium Cyanate Induces Apoptosis of Human Colorectal Cancer Cell via Mitochondrial Pathway
|
|
Yang Eun-Ju
Chang Jeong-Hyun
|
|
|
Abstract
|
|
|
|
Potassium cyanate (KOCN) is an inorganic compound and induces the carbamylation of proteins with cytotoxic effects on human cells. Although there is a potential cytotoxic molecule, the role of KOCN on the apoptosis of cancer cell is not well understood. The present study investigated the effects of KOCN on the human colorectal cancer cell line, HCT 116 cells. To understand the anti-cancer effect of KOCN on HCT 116 cells, we examined alteration of apoptosis, the intracellular Ca2+ concentration, the intracellular signaling pathway and generation of reactive oxygen species (ROS) in these cells treated with KOCN. The apoptosis of HCT 116 cells was induced by KOCN in a dose-dependent manner at 24 hours and 48 hours, respectively. The apoptosis was processed via the cleavage of poly ADP-ribose polymerase (PARP) and activation of caspase 3 in HCT 116 cells. KOCN induced the elevation of intracellular Ca2+ concentration and changed the expressions of Bcl-2 family proteins. The pro-apoptotic Bax was continuously up-regulated, and the anti-apoptotic Bcl-2 was down-regulated by KOCN. KOCN also induced the hyperpolarization of mitochondria and the generation of ROS in HCT 116 cells. Taken together, these results indicate that KOCN induces the apoptosis of HCT 116 cells by disruption of Ca2+ homeostasis and via mitochondrial pathway. This study provides the compound that may be used as a potent agent for the treatment of colorectal cancer.
|
|
|
KEYWORD
|
|
|
Potassium cyanate, Apoptosis, Colorectal cancer, Mitochondrial pathway, Ca2+
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|